Seminars in Perinatology
Volume 34, Issue 2 , Pages 163-169 , April 2010

Digital Microfluidics: A Future Technology in the Newborn Screening Laboratory?

  • David S. Millington, PhD

      Affiliations

    • Department of Pediatrics, Duke University Medical Center, Durham, NC
    • Corresponding Author InformationAddress reprint requests to David S. Millington, PhD, Department of Pediatrics, Duke University Medical Center, Biochemical Genetics Laboratory, 801–6 Capitola Drive, Durham, NC 27713
    • ,
  • Ramakrishna Sista, PhD

      Affiliations

    • Advanced Liquid Logic, Inc., Research Triangle Park, NC
    • ,
  • Allen Eckhardt, PhD

      Affiliations

    • Advanced Liquid Logic, Inc., Research Triangle Park, NC
    • ,
  • Jeremy Rouse

      Affiliations

    • Advanced Liquid Logic, Inc., Research Triangle Park, NC
    • ,
  • Deeksha Bali, PhD

      Affiliations

    • Department of Pediatrics, Duke University Medical Center, Durham, NC
    • ,
  • Ronald Goldberg, MD

      Affiliations

    • Department of Pediatrics, Duke University Medical Center, Durham, NC
    • ,
  • Michael Cotten, MD

      Affiliations

    • Department of Pediatrics, Duke University Medical Center, Durham, NC
    • ,
  • Rebecca Buckley, MD

      Affiliations

    • Department of Pediatrics, Duke University Medical Center, Durham, NC
    • ,
  • Vamsee Pamula, PhD

      Affiliations

    • Advanced Liquid Logic, Inc., Research Triangle Park, NC

References 

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  3. MacCready RA, Hussey MG. Newborn phenylketonuria detection program in Massachusetts. Am J Public Health. 1964;54:2075–2081
  4. Millington DS, Kodo N, Norwood DL, et al. Tandem mass spectrometry: a new method for acylcarnitine profiling with potential for neonatal screening for inborn errors of metabolism. J Inherit Metab Dis. 1990;13:321–324
  5. Rashed MS, Bucknall MP, Little D, et al. Screening blood spots for inborn errors of metabolism by electrospray tandem mass spectrometry with a microplate batch process and a computer algorithm for automated flagging of abnormal profiles. Clin Chem. 1997;43:1129–1141
  6. Chace DH, Kallas TA, Naylor EW. The application of tandem mass spectrometry to neonatal screening for inherited disorders of intermediary metabolism. Annu Rev Genomics Hum Genet. 2002;3:17–45
  7. Desnick RJ. Enzyme replacement and enhancement therapies for lysosomal diseases. J Inherit Metab Dis. 2004;27:385–410
  8. Millington DS. Rapid and effective screening for lysosomal storage disease: How close are we?. Clin Chem. 2008;54:1592–1594
  9. Chien YH, Chiang SC, Zhang XK, et al. Early detection of Pompe disease by newborn screening is feasible: results from the Taiwan screening program. Pediatrics. 2008;122:e39–e45
  10. Chan K, Puck JM. Development of population-based newborn screening for severe combined immunodeficiency. J Allergy Clin Immunol. 2005;115:391–399
  11. Baker MW, Grossman WJ, Laessig RH, et al. Development of a routine newborn screening protocol for severe combined immunodeficiency. J Allergy Clin Immunol. 2009;124:522–527
  12. Bailey DB. Newborn screening for fragile X syndrome. Ment Retard Dev Disabil Res Rev. 2004;10:3–10
  13. Tassone F, Pan R, Amiri K, et al. A rapid polymerase chain reaction-based screening method for identification of all expanded alleles of the fragile X (FMR1) gene in newborn and high-risk populations. J Mol Diagn. 2008;10:43–49
  14. Modell B, Darr A. Science and society: Genetic counselling and customary consanguineous marriage. Nat Rev Genet. 2002;3:225–229
  15. Pollack MG, Shenderov AD, Fair RB. Electrowetting-based actuation of droplets for integrated microfluidics. Lab Chip. 2002;2:96–101
  16. Paik P, Pamula VK, Pollack MG, et al. Electrowetting-based droplet mixers for microfluidic systems. Lab Chip. 2003;3:28–33
  17. Sista R, Hua Z, Thwar P, et al. Development of a digital microfluidic platform for point of care testing. Lab Chip. 2008;8:2091–2104
  18. Srinivasan V, Pamula VK, Fair RB. An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human physiological fluids. Lab Chip. 2004;4:310–315
  19. Sista RS, Eckhardt AE, Srinivasan V, et al. Heterogeneous immunoassays using magnetic beads on a digital microfluidic platform. Lab Chip. 2008;8:2188–2196
  20. Gelb MH, Turecek F, Scott CR, et al. Direct multiplex assay of enzymes in dried blood spots for the newborn screening of lysosomal storage disorders. J Inherit Metab Dis. 2006;29:397–404
  21. Rouse J, Eckhardt AE, Millington DS, et al. Digital microfluidics: a novel platform for multiplexing assays used in newborn screening [abstract]. Clin Chem Data Suppl. 2009;55:1891
  22. Broda E, Baumgartner ER, Scholl S, et al. Biotinidase determination in serum and dried blood spots—High sensitivity fluorimetric ultramicro-assay. Clin Chim Acta. 2001;314:175–185
  23. Sontag MK, Corey M, Hokanson JE, et al. Genetic and physiologic correlates of longitudinal immunoreactive trypsinogen decline in infants with cystic fibrosis identified through newborn screening. J Pediatr. 2006;149:650–657
  24. Maisels MJ, Bhutani VK, Bogen D, et al. Hyperbilirubinemia in the newborn infant > or =35 weeks' gestation: an update with clarifications. Pediatrics. 2009;124:1193–1198
  25. Newman TB. Universal bilirubin screening, guidelines, and evidence. Pediatrics. 2009;124:1199–1202
  26. Kaplan M, Hammerman C: Glucose-6-phosphate dehydrogenase deficiency and severe neonatal hyperbilirubinemia: a complexity of interactions between genes and environment. Semin Fetal Neonatal Med (in press)

 Partially supported by Award Number R44HD057713 from the Eunice Kennedy Shriver National Institute of Child Health and Human Development. Funding was also provided by the Jean and George Brumley Jr, Neonatal-Perinatal Research Institute.

 The content is solely the responsibility of the authors and does not necessarily represent the official views of the Eunice Kennedy Shriver National Institute of Child Health and Human Development or the National Institutes of Health.

PII: S0146-0005(09)00113-X

doi: 10.1053/j.semperi.2009.12.008

Seminars in Perinatology
Volume 34, Issue 2 , Pages 163-169 , April 2010

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